A trial of Rappaport's medium.

Rappaport's medium was tried out in parallel with, and in combination with, tetrathionate broth, and selenite F medium in 1,000 consecutive stool cultures. Media. Selenite F medium, tetrathionate broth, and desoxycholate-citrate-agar (D.C.A.) were made according to the formulae by Mackie and McCartney (1953). Rappaport's medium was prepared according to the formula given by Rappaport et a[. (1956). In order to make allowance for the effect of inoculating more bottles, and to test the effect of previous dilution of faeces upon the efficacy of each of the selective media, each specimen was inoculated into all three media both with a small inoculum, that is, 5 drops of a 1:1,000 suspension of faeces in saline, and with a large inoculum, a particle of faeces as big